低盐状态下的聚（dA-dT）·聚（dA-dT）似乎是一种左旋B型螺旋，它综合运用了化学理论、纤维衍射和核磁共振光谱学。
Poly(dA-dT).poly(dA-dT) in low salt appears to be a left-handed B-helix combined use of chemical theory, fiber diffraction and NMR spectroscopy.
作者信息
Gupta G, Sarma M H, Dhingra M M, Sarma R H, Rajagopalan M, Sasisekharan V
机构信息
Institute of Biomolecular Stereodynamics, State University of New York, Albany 12222.
出版信息
J Biomol Struct Dyn. 1983 Oct;1(2):395-416. doi: 10.1080/07391102.1983.10507450.
Poly(dA-dT).poly(dA-dT) can adopt the B- and D- forms in the fibrous state. Theoretical energy calculations and fiber diffraction analyses suggest that there can be three structural models of poly(dA-dT).poly(dA-dT) in each of these two forms viz right and left-handed Watson Crick models and left-handed Hoogsteen--a total of six possible models. Fiber data for the polymer in the B- or the D-form or energy calculations cannot distinguish any one model from the other. However, a comparison of observed proton chemical shifts with the theoretically computed ones and the NOE studies on exchangeable and nonexchangeable protons suggest that poly(dA-dT).poly(dA-dT) in low salt solution exists predominantly in the left-handed B-conformation.
聚（dA-dT）·聚（dA-dT）在纤维状态下可以呈现B型和D型。理论能量计算和纤维衍射分析表明，在这两种形式中，聚（dA-dT）·聚（dA-dT）各有三种结构模型，即右手和左手沃森-克里克模型以及左手霍格斯坦模型，总共六种可能的模型。B型或D型聚合物的纤维数据或能量计算无法区分任何一种模型。然而，将观察到的质子化学位移与理论计算值进行比较，以及对可交换和不可交换质子的核Overhauser效应（NOE）研究表明，低盐溶液中的聚（dA-dT）·聚（dA-dT）主要以左手B构象存在。
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