Pierotti M A, Traversari C, Ballinari D, Borrello M G, Parmiani G
Division of Experimental Oncology A, National Tumor Institute, Milan, Italy.
Exp Clin Immunogenet. 1984;1(2):75-82.
Four different molecular weight forms of the Ly-5 antigen were defined by immunochemical methods on the cell surface of murine spleen cells using a monoclonal antibody. Each form was selectively expressed by cells belonging to different lineages. A 220,000-dalton molecule was associated with B cells, a 200,000-dalton molecule with macrophages and two antigens of 185,000 and 175,000 daltons, respectively, were detected on the cell surface of normal thymocytes. The 185,000-dalton Ly-5 molecule was not found on the lymphoid cells of immunodeficient mice, like the athymic nude or the athymic-asplenic (LASAT) mice, suggesting that cells bearing this Ly-5 molecule have a significant function in some T-dependent immune responses.
利用单克隆抗体，通过免疫化学方法在小鼠脾细胞的细胞表面确定了Ly-5抗原的四种不同分子量形式。每种形式由属于不同谱系的细胞选择性表达。一个220,000道尔顿的分子与B细胞相关，一个200,000道尔顿的分子与巨噬细胞相关，在正常胸腺细胞的细胞表面分别检测到185,000和175,000道尔顿的两种抗原。在免疫缺陷小鼠（如无胸腺裸鼠或无胸腺无脾（LASAT）小鼠）的淋巴细胞上未发现185,000道尔顿的Ly-5分子，这表明携带这种Ly-5分子的细胞在某些T细胞依赖性免疫反应中具有重要功能。
