Immunological cross-reactivity of mouse alcohol dehydrogenase with rabbit immune sera against human alcohol dehydrogenase isozymes.

Rabbit immune sera against human alcohol dehydrogenase (ADH) isozymes were found to cross-react with mouse ADH. The immune serum raised against horse ADH and specific for the human isozymes coded at the ADH1, ADH2 and ADH3 loci (class I of ADH isozymes) reacted with the ADH-A2 isozyme from mouse liver and ADH-C2 isozyme from mouse stomach. The two mouse isozymes exhibit partial immunological identity. The immune serum raised against the human chi-ADH isozyme coded at the ADH5 locus (class III of ADH isozymes) reacted with the ADH-B2 isozyme from mouse stomach and liver. The results indicate that the A2 and C2 forms of mouse ADH may be related to the human ADH isozymes coded at the ADH1, ADH2 and ADH3 loci whereas the B2 form may be homologous of the human ADH isozyme coded at the ADH5 locus.