Nevalainen T J
Exp Cell Biol. 1978;46(4):193-8. doi: 10.1159/000162894.
Quantitative changes in the pancreatic acinar cell organelles were studied in BALB/c mice injected with 1.0 ml fresh rabbit serum intraperitoneally. Groups of 5 mice were killed at 0, 1, 3, 6 and 12 h after the serum injection. Pancreatic tissue was processed for electron microscopy by glutaraldehyde and osmium tetroxide fixation and Epon embedding. The proportions of acinar cell cytoplasm (volume fractions) occupied by zymogen granules, granular endoplasmic reticulum, Golgi apparatus, mitochondria and lysosomes (including autophagosomes) were determined by the point counting method from electron micrographs. The volume fraction of lysosomes increased during the first 3 h and remained markedly elevated up to 12 h. The volume fractions of zymogen granules increased from 12 to 28% in 12 h. It was concluded that the secretory mechanism of pancreatic acinar cells was injured by the foreign serum. The injury caused accumulation of zymogen granules and increased autophagic activity in the acinar cells.
对腹腔注射1.0 ml新鲜兔血清的BALB/c小鼠胰腺腺泡细胞细胞器的定量变化进行了研究。在血清注射后的0、1、3、6和12小时处死每组5只小鼠。胰腺组织经戊二醛和四氧化锇固定、环氧树脂包埋后进行电子显微镜处理。通过电子显微镜照片的点计数法确定酶原颗粒、颗粒内质网、高尔基体、线粒体和溶酶体（包括自噬体）所占腺泡细胞质的比例（体积分数）。溶酶体的体积分数在前3小时增加，并在12小时内一直显著升高。酶原颗粒的体积分数在12小时内从12%增加到28%。得出的结论是，外来血清损伤了胰腺腺泡细胞的分泌机制。这种损伤导致酶原颗粒的积累和腺泡细胞自噬活性的增加。
