人红细胞丁醇提取物的H特异性
The H specificities of butanol extracts of human erythrocytes.
作者信息
Lambert R M, Zelenski S K
出版信息
Vox Sang. 1976;31(1 SUPPL):77-83. doi: 10.1111/j.1423-0410.1976.tb02225.x.
A previous report of other researchers that aqueous phase fractions of erythrocyte stromata of secretors contained more ABH blood group activity than similar fractions of non-secretors was confirmed. The recovery of H activity in the butanol phase extracts of both secretors and non-secretors, which had not been seen previously, is reported here for the first time. Three H specificities were present when the aqueous and butanol phases of group O secretor stromata were examined in inhibition of agglutination studies with anti-H of group Oh and group A1 sera and three lectins. The anti-H (group Oh) sera were inhibited by both aqueous and butanol fractions. Anti-H serum (group A1) and the lectin Ulex europaeus were inhibited by the aqueous fraction only. The lectins Laburnum alpinum and Lotus tetragonolobus were not inhibited by either the aqueous or butanol phase extracts.
其他研究人员之前的一份报告得到了证实，该报告称分泌型红细胞基质的水相部分比非分泌型的类似部分含有更多的ABH血型活性。本文首次报道了分泌型和非分泌型的丁醇相提取物中H活性的回收情况，这是之前未曾见过的。在用Oh组和A1组抗-H血清以及三种凝集素进行的凝集抑制研究中，检测O组分泌型基质的水相和丁醇相时，发现了三种H特异性。Oh组抗-H血清被水相和丁醇相部分均抑制。A1组抗-H血清和欧洲荆豆凝集素仅被水相部分抑制。高山金链花凝集素和四角百脉根凝集素均未被水相或丁醇相提取物抑制。
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