Safa A R, Tseng M T
Experientia. 1983 Mar 15;39(3):283-5. doi: 10.1007/BF01955306.
Heterogeneity of cells in a solid tumor prevents direct assessment of enzyme activity of cell subpopulations by conventional homogenization techniques. Lactate dehydrogenase (LDH) activity before and after ovariectomy and following estrogen supplementation in density-defined cell subpopulations from DMBA-induced mammary tumors was determined. Different levels of LDH activity were found in different cell subpopulations. After ovariectomy the level of LDH activity declined. Restoration of the circulatory estrogen level resulted in increased enzyme activity. The highest level of LDH was found in a band which consisted mainly of poorly differentiated cells. This cell subpopulation also tended to be more responsive to endocrine manipulation.
实体瘤中细胞的异质性使得无法通过传统的匀浆技术直接评估细胞亚群的酶活性。我们测定了DMBA诱导的乳腺肿瘤中密度定义的细胞亚群在卵巢切除术前、术后以及雌激素补充后的乳酸脱氢酶（LDH）活性。在不同的细胞亚群中发现了不同水平的LDH活性。卵巢切除术后，LDH活性水平下降。循环雌激素水平的恢复导致酶活性增加。在一个主要由低分化细胞组成的条带中发现了最高水平的LDH。这个细胞亚群对内分泌操纵也往往更敏感。
