Gaasterland D E, Barranger J A, Rapoport S I, Girton M E, Doppman J L
Invest Ophthalmol Vis Sci. 1983 Feb;24(2):153-8.
The blood-aqueous humor barrier in adult rhesus monkeys was opened by intracarotid infusion of a 25% mannitol solution. Each monkey had one to four infusions into the same artery, with at least one week between subsequent procedures. The monkeys were observed clinically for 3 to 212 days. Within an hour pigmented cells and protein accumulated in the aqueous humor of the mannitol-treated eyes. Hypotony developed within a day. Aqueous flare and cells cleared within 2 weeks. Hypotony resolved in 8 to 12 weeks. More than one half of the monkeys had transient anisocoria. In some, the pupil in the treated eye was miotic; in others it was dilated. Direct and consensual pupil responses to light remained intact in untreated eyes and in treated eyes with mydriasis. About one fourth of the monkeys developed edema of the ipsilateral optic disk. This correlated with hypotony. No monkey developed cataract, corneal opacity, or vitreous or retinal change. The aqueous protein concentration was slightly above normal a month or more after the carotid infusions, but was considerably less than plasma protein concentration. Posterior and anterior aqueous ascorbate concentrations in treated eyes were slightly below normal, but far greater than plasma concentration, indicating that ascorbate active transport by the ciliary epithelium was essentially intact despite the widespread, permanent structural alteration that had been caused by the mannitol treatment.
通过颈内动脉输注25%的甘露醇溶液来打开成年恒河猴的血-房水屏障。每只猴子在同一动脉进行1至4次输注，后续操作之间至少间隔一周。对猴子进行了3至212天的临床观察。在一小时内，色素细胞和蛋白质在甘露醇处理眼的房水中积聚。一天内出现低眼压。房水闪光和细胞在2周内清除。低眼压在8至12周内消退。超过一半的猴子出现短暂性瞳孔不等大。在一些猴子中，处理眼的瞳孔缩小；在另一些猴子中，瞳孔散大。未处理眼以及瞳孔散大的处理眼对光的直接和间接瞳孔反应仍保持完整。约四分之一的猴子出现同侧视盘水肿。这与低眼压相关。没有猴子出现白内障、角膜混浊或玻璃体或视网膜改变。在颈动脉输注后一个月或更长时间，房水蛋白质浓度略高于正常水平，但远低于血浆蛋白质浓度。处理眼的房水后段和前段抗坏血酸浓度略低于正常水平，但远高于血浆浓度，这表明尽管甘露醇处理已导致广泛的永久性结构改变，但睫状体上皮对抗坏血酸的主动转运基本完好。
