运用定量免疫荧光技术鉴别炭疽芽孢杆菌和蜡样芽孢杆菌的孢子。
Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique.
作者信息
Phillips A P, Martin K L, Broster M G
出版信息
J Clin Microbiol. 1983 Jan;17(1):41-7. doi: 10.1128/jcm.17.1.41-47.1983.
Abstract
A quantitative immunofluorescence assay based on fiber optic microscopy was used to measure the reaction of formalized spores of Bacillus anthracis and Bacillus cereus isolates with fluorescein conjugates prepared by hyperimmunization with B. anthracis Vollum spores. The spores of 11 of the 20 B. cereus strains reacted with the anti-anthrax conjugate to such an extent that they were indistinguishable from the spores of the several B. anthracis isolates tested. However, absorption of the conjugate with spores of B. cereus NCTC 8035 and B. cereus NCTC 10320 greatly reduced the cross-reaction with the B. cereus preparations so that the mean specific fluorescence of samples of B. cereus spores was in no case higher than 14% of the fluorescence of a reference B. anthracis Vollum preparation.
摘要
基于光纤显微镜的定量免疫荧光测定法被用于测量炭疽芽孢杆菌和蜡样芽孢杆菌分离株的甲醛化孢子与用炭疽芽孢杆菌Vollum孢子超免疫制备的荧光素缀合物的反应。20株蜡样芽孢杆菌菌株中的11株的孢子与抗炭疽缀合物发生反应，其程度与所测试的几种炭疽芽孢杆菌分离株的孢子无法区分。然而，用蜡样芽孢杆菌NCTC 8035和蜡样芽孢杆菌NCTC 10320的孢子吸收缀合物大大降低了与蜡样芽孢杆菌制剂的交叉反应，使得蜡样芽孢杆菌孢子样品的平均特异性荧光在任何情况下都不高于参考炭疽芽孢杆菌Vollum制剂荧光的14%。
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