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Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique.运用定量免疫荧光技术鉴别炭疽芽孢杆菌和蜡样芽孢杆菌的孢子。
J Clin Microbiol. 1983 Jan;17(1):41-7. doi: 10.1128/jcm.17.1.41-47.1983.
2
Investigation of spore surface antigens in the genus Bacillus by the use of polyclonal antibodies in immunofluorescence tests.利用多克隆抗体通过免疫荧光试验对芽孢杆菌属中的孢子表面抗原进行研究。
J Appl Bacteriol. 1988 Jan;64(1):47-55. doi: 10.1111/j.1365-2672.1988.tb02428.x.
3
Identification of Bacillus anthracis by polyclonal antibodies against extracted vegetative cell antigens.利用针对提取的营养细胞抗原的多克隆抗体鉴定炭疽芽孢杆菌。
J Appl Bacteriol. 1989 May;66(5):419-32. doi: 10.1111/j.1365-2672.1989.tb05111.x.
4
Quantitative immunofluorescence studies of the serology of Bacillus anthracis spores.炭疽芽孢杆菌孢子血清学的定量免疫荧光研究。
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Evaluation of a microfluorometer in immunofluorescence assays of individual spores of Bacillus anthracis and Bacillus cereus.微量荧光计在炭疽芽孢杆菌和蜡样芽孢杆菌单个孢子免疫荧光测定中的评估
J Immunol Methods. 1982 Mar 26;49(3):271-82. doi: 10.1016/0022-1759(82)90127-2.
6
Secondary cell wall polysaccharides of Bacillus anthracis are antigens that contain specific epitopes which cross-react with three pathogenic Bacillus cereus strains that caused severe disease, and other epitopes common to all the Bacillus cereus strains tested.炭疽芽孢杆菌的次生细胞壁多糖是抗原，其包含与三株引起严重疾病的致病性蜡样芽孢杆菌菌株发生交叉反应的特定表位，以及所有测试的蜡样芽孢杆菌菌株共有的其他表位。
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7
Significant passive protective effect against anthrax by antibody to Bacillus anthracis inactivated spores that lack two virulence plasmids.针对缺乏两个毒力质粒的炭疽芽孢杆菌灭活孢子的抗体对炭疽具有显著的被动保护作用。
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Immunization of mice with formalin-inactivated spores from avirulent Bacillus cereus strains provides significant protection from challenge with Bacillus anthracis Ames.用来自无毒蜡状芽孢杆菌菌株的福尔马林灭活孢子对小鼠进行免疫，可提供针对炭疽芽孢杆菌埃姆斯菌株攻击的显著保护。
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Proteomic profiling and identification of immunodominant spore antigens of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis.炭疽芽孢杆菌、蜡样芽孢杆菌和苏云金芽孢杆菌的蛋白质组分析及免疫显性孢子抗原鉴定
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Identification of Bacillus anthracis spore component antigens conserved across diverse Bacillus cereus sensu lato strains.跨多种蜡样芽胞杆菌复合群菌株保守的炭疽芽孢杆菌孢子成分抗原的鉴定。
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引用本文的文献

1
Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.异质微生物群体的流式细胞术和细胞分选：单细胞分析的重要性。
Microbiol Rev. 1996 Dec;60(4):641-96. doi: 10.1128/mr.60.4.641-696.1996.
2
Glycosidase activities of Bacillus anthracis.炭疽芽孢杆菌的糖苷酶活性
J Clin Microbiol. 1984 May;19(5):594-8. doi: 10.1128/jcm.19.5.594-598.1984.
3
Quantitative immunofluorescence studies of the serology of Bacillus anthracis spores.炭疽芽孢杆菌孢子血清学的定量免疫荧光研究。
Appl Environ Microbiol. 1983 Dec;46(6):1430-2. doi: 10.1128/aem.46.6.1430-1432.1983.
4
Differentiation of Bacillus anthracis and other Bacillus species by lectins.通过凝集素鉴别炭疽芽孢杆菌和其他芽孢杆菌属物种。
J Clin Microbiol. 1984 Jan;19(1):48-53. doi: 10.1128/jcm.19.1.48-53.1984.

本文引用的文献

1
Bacterial spore antigens: a review.细菌芽孢抗原：综述
J Gen Microbiol. 1962 Jul;28:393-408. doi: 10.1099/00221287-28-3-393.
2
Comparative study of the agglutinogens of the endospores of Bacillus anthracis and Bacillus cereus.炭疽芽孢杆菌和蜡样芽孢杆菌芽孢凝集原的比较研究
J Bacteriol. 1960 Mar;79(3):435-41. doi: 10.1128/jb.79.3.435-441.1960.
3
Intrinsically stable IgG aggregates.内在稳定的IgG聚集体。
J Immunol Methods. 1981;41(1):63-74. doi: 10.1016/0022-1759(81)90274-x.
4
Evaluation of a microfluorometer in immunofluorescence assays of individual spores of Bacillus anthracis and Bacillus cereus.微量荧光计在炭疽芽孢杆菌和蜡样芽孢杆菌单个孢子免疫荧光测定中的评估
J Immunol Methods. 1982 Mar 26;49(3):271-82. doi: 10.1016/0022-1759(82)90127-2.
5
Agar diffusion method for the differentiation of Bacillus anthracis.用于鉴别炭疽芽孢杆菌的琼脂扩散法。
Appl Microbiol. 1971 Jan;21(1):157-9. doi: 10.1128/am.21.1.157-159.1971.
6
[A fluorescence serological rapid test for the determination of spores from Bacillus anthracis using the micro culture method on nucleopore filters].[一种采用核孔滤膜微量培养法测定炭疽芽孢杆菌孢子的荧光血清学快速检测方法]
Zentralbl Veterinarmed B. 1974 May;21(5):329-35.
7
Rapid fluorescein and protein assay method for fluorescent-antibody conjugates.荧光抗体结合物的快速荧光素和蛋白质测定方法。
Appl Microbiol. 1966 Mar;14(2):271-5. doi: 10.1128/am.14.2.271-275.1966.
8
[Fluorescent serologic studies on cross reactions between spores of Bacillus anthracis and spores of other aerobic sporeforming bacteria].
Zentralbl Veterinarmed B. 1977 Jun;24(6):497-507.
9
Specific staining of bacilli by means of immuno-indian-ink method and immunofluorescent method.通过免疫墨汁法和免疫荧光法对杆菌进行特异性染色。
Zentralbl Bakteriol Orig A. 1979 Mar;243(1):119-24.

运用定量免疫荧光技术鉴别炭疽芽孢杆菌和蜡样芽孢杆菌的孢子。

Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique.

作者信息

Phillips A P, Martin K L, Broster M G

出版信息

J Clin Microbiol. 1983 Jan;17(1):41-7. doi: 10.1128/jcm.17.1.41-47.1983.

DOI:10.1128/jcm.17.1.41-47.1983
PMID:6402519
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272570/
Abstract

A quantitative immunofluorescence assay based on fiber optic microscopy was used to measure the reaction of formalized spores of Bacillus anthracis and Bacillus cereus isolates with fluorescein conjugates prepared by hyperimmunization with B. anthracis Vollum spores. The spores of 11 of the 20 B. cereus strains reacted with the anti-anthrax conjugate to such an extent that they were indistinguishable from the spores of the several B. anthracis isolates tested. However, absorption of the conjugate with spores of B. cereus NCTC 8035 and B. cereus NCTC 10320 greatly reduced the cross-reaction with the B. cereus preparations so that the mean specific fluorescence of samples of B. cereus spores was in no case higher than 14% of the fluorescence of a reference B. anthracis Vollum preparation.

摘要

基于光纤显微镜的定量免疫荧光测定法被用于测量炭疽芽孢杆菌和蜡样芽孢杆菌分离株的甲醛化孢子与用炭疽芽孢杆菌Vollum孢子超免疫制备的荧光素缀合物的反应。20株蜡样芽孢杆菌菌株中的11株的孢子与抗炭疽缀合物发生反应，其程度与所测试的几种炭疽芽孢杆菌分离株的孢子无法区分。然而，用蜡样芽孢杆菌NCTC 8035和蜡样芽孢杆菌NCTC 10320的孢子吸收缀合物大大降低了与蜡样芽孢杆菌制剂的交叉反应，使得蜡样芽孢杆菌孢子样品的平均特异性荧光在任何情况下都不高于参考炭疽芽孢杆菌Vollum制剂荧光的14%。