Nakayama H, Goto M, Ohki K, Mitsui T, Nozawa Y
Biochim Biophys Acta. 1983 Apr 21;730(1):17-24. doi: 10.1016/0005-2736(83)90311-5.
Mitochondrial, microsomal and pellicular membranes were isolated from Tetrahymena cells grown at 39 degrees C or 15 degrees C, and phospholipids, in turn, were separated from total lipids extracted from these membranes. The effect of growth temperature on their solid-to-fluid phase transition temperature was examined by wide-angle X-ray diffraction. The transition temperatures of phospholipids from mitochondria, microsomes and pellicles were 21, 19 and 26 degrees C for cells grown at 39 degrees C and -8, -3 and 6 degrees C for cells grown at 15 degrees C, respectively. All phospholipids were found in a completely fluid state at these growth temperatures. From a comparison between the phospholipids and total lipids from pellicles of cells grown at 39 degrees C, a triterpenoid alcohol, tetrahymanol, caused the transition temperature to increase. The alignment of tetrahymanol in membranes was examined with pellicle'a total lipid oriented in a sample holder.
从在39℃或15℃下生长的四膜虫细胞中分离出线粒体膜、微粒体膜和表膜，然后从这些膜中提取的总脂质中分离出磷脂。通过广角X射线衍射研究生长温度对其固-液相转变温度的影响。对于在39℃下生长的细胞，线粒体、微粒体和表膜中磷脂的转变温度分别为21℃、19℃和26℃；对于在15℃下生长的细胞，相应的转变温度分别为-8℃、-3℃和6℃。在这些生长温度下，所有磷脂均处于完全流动状态。通过比较39℃下生长的细胞表膜中的磷脂和总脂质，发现一种三萜醇——四膜虫醇会导致转变温度升高。使用固定在样品架中的表膜总脂质来研究四膜虫醇在膜中的排列情况。
