未成熟和成熟鸽红细胞中富含丝氨酸的组蛋白（H5、F2c）之间阻遏物特性存在差异。

Existence of differences in repressor properties between serine-rich histones (H5, F2c) from immature and mature pigeon erythroid cells.

作者信息

Gasaryan K G, Andreeva N B, Vishnevskaya T Y

出版信息

Differentiation. 1978 Mar 13;10(2):123-7. doi: 10.1111/j.1432-0436.1978.tb00953.x.

DOI:10.1111/j.1432-0436.1978.tb00953.x

Abstract

The abilities of H1 and H5 histones from immature and mature pigeon erythroid cells and subfractions of H5 histones with different content of alkali-labile phosphorus to restrict RNA synthesis were compared in an in vitro transcription system with bacterial RNA polymerase. It has been found that: a) H1 histones from both sources exhibit similar efficiency. b) H5 histones from immature cells restrict transcription to a lesser extent than the same histone from the mature erythrocyte. c) The subfraction of H5 histone from immature cells with the higher content of alkali-labile phosphorus restrict transcription less than the subfraction with the lower degree of phosphorylation. The results suggest that H5 histone, which first appears in the erythroblasts in a highly phosphorylated form, does not display its potential 'repressor' properties. During erythrocyte maturation H5 histone is dephosphorylated and this causes the massive inactivation of erythrocyte genome.

摘要

在含有细菌RNA聚合酶的体外转录系统中，比较了来自未成熟和成熟鸽红细胞的H1和H5组蛋白以及具有不同碱不稳定磷含量的H5组蛋白亚组分限制RNA合成的能力。结果发现：a）来自两种来源的H1组蛋白表现出相似的效率。b）未成熟细胞中的H5组蛋白比成熟红细胞中的相同组蛋白对转录的限制程度小。c）来自未成熟细胞的碱不稳定磷含量较高的H5组蛋白亚组分比磷酸化程度较低的亚组分对转录的限制更少。结果表明，首先以高度磷酸化形式出现在成红细胞中的H5组蛋白并未表现出其潜在的“阻遏物”特性。在红细胞成熟过程中，H5组蛋白去磷酸化，这导致红细胞基因组大量失活。