从兔肝脏中分离支链α-酮酸脱氢酶。
Isolation of a branched chain alpha-keto acid dehydrogenase from rabbit liver.
作者信息
Morrison E Y, Mullings K
出版信息
Biochim Biophys Acta. 1983 Jan 25;755(2):225-8. doi: 10.1016/0304-4165(83)90207-6.
PMID:6403047
Abstract
An enzyme catalysing a series of reactions resulting in the oxidative decarboxylation of branched chain alpha-keto acids and production of NADH, was extracted from rabbit liver mitochondria with the aid of NaClO4. Purification yielded a product which appeared homogeneous on electrophoresis. The enzyme is active on three substrates alpha-ketoisocaproate, alpha-keto-beta-methyl valerate, and alpha-ketoisovalerate.
摘要
借助高氯酸钠从兔肝线粒体中提取出一种酶，该酶催化一系列反应，导致支链α-酮酸的氧化脱羧并产生NADH。纯化后得到的产物在电泳中显示为单一成分。该酶对三种底物α-酮异己酸、α-酮-β-甲基戊酸和α-酮异戊酸具有活性。
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