嗜碱芽孢杆菌的DNA可将枯草芽孢杆菌转化为嗜碱性。

DNA from alkalophilic Bacillus can transform B. subtilis to alkalophily.

作者信息

Takinishi H, Sekiguchi T, Koyama N, Shishido K, Nosoh Y

出版信息

FEBS Lett. 1983 Apr 5;154(1):201-4. doi: 10.1016/0014-5793(83)80903-x.

Abstract

On incubation of B. subtilis RM125(arg 15 leuA8 rM- mM-) with DNA from alkalophilic Bacillus, the transformants (Arg+Leu- or Leu-Arg+) appeared at pH 10. The transformants were able to grow even at pH 7. Alkalophilic Bacillus was resistant to bacteriophages ø105D1C2.1012 grown on B. subtilis 1012(r-mM+) and ø105D1C2.ISMR4 grown on B. subtilis ISMR4(rM+rR+mM+mR+), but the recipient B. subtilis and the transformant(Arg+Leu-) were susceptible to both of the bacteriophages. The results indicate that the transformant is a B. subtilis derivative and that alkalophilicity of alkalophilic Bacillus was transferred to B. subtilis.

摘要

将枯草芽孢杆菌RM125（arg 15 leuA8 rM- mM-）与嗜碱芽孢杆菌的DNA一起培养时，转化体（Arg + Leu-或Leu-Arg +）在pH 10时出现。这些转化体甚至在pH 7时也能生长。嗜碱芽孢杆菌对在枯草芽孢杆菌1012（r-mM +）上生长的噬菌体ø105D1C2.1012和在枯草芽孢杆菌ISMR4（rM + rR + mM + mR +）上生长的噬菌体ø105D1C2.ISMR4具有抗性，但受体枯草芽孢杆菌和转化体（Arg + Leu-）对这两种噬菌体均敏感。结果表明，转化体是枯草芽孢杆菌的衍生物，嗜碱芽孢杆菌的嗜碱性已转移到枯草芽孢杆菌中。