Fonty G, Gouet P, Jouany J P, Senaud J
J Gen Microbiol. 1983 Jan;129(1):213-23. doi: 10.1099/00221287-129-1-213.
Ecological factors that control the establishment of cellulolytic bacteria and ciliate protozoa in the lamb rumen were studied in meroxenic animals. Axenic lambs received dilutions of rumen liquor from either conventional lambs and sheep (pool A) or meroxenic lambs (pool B). The total number of bacteria established in the rumen was between 10(9) and 5 x 10(10) g-1. In lambs inoculated with dilutions (10(-6), 10(-7), 10(-8)) of pool A, cellulolytic bacteria did not become established. However, subsequent inoculation with Bacteroides succinogenes, resulted in colonization in lambs that had received 10(-6) and 10(-7) dilutions of pool A. However, B. succinogenes became established in only one of three lambs that received the 10(-8) dilution. Similar results were obtained for the protozoan Entodinium sp. With pool B, lambs were inoculated earlier and cellulolytic bacteria were established directly from the 10(-6) and 10(-7) inocula. Polyplastron multivesiculatum establishment occurred readily when inoculated into the lambs which had received the 10(-6) dilution of pool B. Results obtained in this study suggest that establishment of cellulolytic bacteria and protozoa requires an abundant and complex flora and is favoured by early animal inoculation.
在无菌动物中研究了控制羔羊瘤胃中纤维素分解菌和纤毛虫原生动物定植的生态因素。无菌羔羊接受来自常规羔羊和绵羊（A组）或无菌羔羊（B组）的瘤胃液稀释液。瘤胃中定植的细菌总数在10⁹至5×10¹⁰ g⁻¹之间。在接种A组稀释液（10⁻⁶、10⁻⁷、10⁻⁸）的羔羊中，纤维素分解菌未定植。然而，随后接种产琥珀酸拟杆菌，导致在接受A组10⁻⁶和10⁻⁷稀释液的羔羊中定植。然而，产琥珀酸拟杆菌仅在接受10⁻⁸稀释液的三只羔羊中的一只中定植。原生动物内毛虫属也得到了类似的结果。对于B组，羔羊接种时间更早，纤维素分解菌直接从10⁻⁶和10⁻⁷接种物中定植。当接种到接受B组10⁻⁶稀释液的羔羊中时，多泡多膜袋虫很容易定植。本研究获得的结果表明，纤维素分解菌和原生动物的定植需要丰富而复杂的菌群，并且早期动物接种有利于其定植。
