Nishibe S, Ogawa M, Murata A, Nakamura K, Hatanaka T, Kambayashi J, Kosaki G
Life Sci. 1983 Apr 4;32(14):1613-20. doi: 10.1016/0024-3205(83)90868-8.
Acetylcholine and arachidonic acid induced catecholamine secretion from isolated bovine adrenal medullary cells. Protease inhibitors and calmodulin inhibitors inhibited catecholamine secretion induced by acetylcholine but did not inhibit the secretion induced by arachidonic acid. BW 755-C, a lipoxygenase inhibitor, inhibited catecholamine release induced by acetylcholine. These results suggest that a protease and calmodulin are involved in the successive reaction after stimulus-receptor coupling and arachidonic acid or its metabolites might be important in catecholamine secretion from bovine adrenal medullary cells.
乙酰胆碱和花生四烯酸可诱导分离的牛肾上腺髓质细胞分泌儿茶酚胺。蛋白酶抑制剂和钙调蛋白抑制剂可抑制乙酰胆碱诱导的儿茶酚胺分泌，但不抑制花生四烯酸诱导的分泌。脂氧合酶抑制剂BW 755-C可抑制乙酰胆碱诱导的儿茶酚胺释放。这些结果表明，蛋白酶和钙调蛋白参与了刺激-受体偶联后的连续反应，花生四烯酸或其代谢产物可能在牛肾上腺髓质细胞分泌儿茶酚胺过程中起重要作用。
