Sato P H, Walton D M
Arch Biochem Biophys. 1983 Mar;221(2):543-7. doi: 10.1016/0003-9861(83)90173-x.
The potential pharmacologic use of enzymes has long been considered. Practical applications, however, have been limited by the toxicity and allergic response to administered foreign proteins. A simple in vitro modification that allows the intraperitoneal administration of large doses of L-gulonolactone oxidase to guinea pigs is described. The enzyme is precipitated by guinea pig antisera and reacted with glutaraldehyde (0.125%). The product is comparatively nontoxic in guinea pigs. Administration of this enzyme enables guinea pigs to synthesize ascorbic acid. Success of this approach may depend on reinforcement by the bifunctional reagent of the enzyme-antibody complex.
酶在药理学上的潜在用途早已受到关注。然而，实际应用一直受到所注入外源蛋白的毒性和过敏反应的限制。本文描述了一种简单的体外修饰方法，该方法可使豚鼠腹腔注射大剂量的L-古洛糖酸内酯氧化酶。该酶用豚鼠抗血清沉淀，然后与戊二醛（0.125%）反应。所得产物在豚鼠中相对无毒。给予这种酶能使豚鼠合成抗坏血酸。这种方法的成功可能取决于双功能试剂对酶-抗体复合物的强化作用。
