Shelt D, Walton D, Sato P
Biomater Med Devices Artif Organs. 1982;10(1):55-62. doi: 10.3109/10731198209118771.
Guinea pigs fed an ascorbic acid-deficient diet develop scurvy because of the absence of the enzyme L-gulonolactone oxidase. In theory if this enzyme is provided and its substrate L-gulonolactone is present at adequate concentrations ascorbic acid will be synthesized and the development of scurvy prevented. Using this model we tested whether a viable segment of intestine could be used to contain the administered enzyme and act as an artificial organ for the production of ascorbic acid. A surgical procedure was developed to prepare an externalized pouch of intestine with its circulation left intact. When enzyme is inserted in this intestinal bag it is not toxic and not antigenic in some animals, whereas, enzyme injected intraperitoneally is clearly antigenic. Synthesis of ascorbic acid by this artificial organ could not, however, be detected by elevation of plasma concentrations of the vitamin.
喂食抗坏血酸缺乏饮食的豚鼠会患坏血病，因为缺乏L-古洛糖酸内酯氧化酶。理论上，如果提供这种酶，并且其底物L-古洛糖酸内酯以足够的浓度存在，抗坏血酸就会合成，坏血病的发展也会得到预防。利用这个模型，我们测试了一段有活力的肠道是否可以用来容纳所给予的酶，并作为产生抗坏血酸的人工器官。我们开发了一种外科手术，以制备一个循环保持完整的外置肠袋。当将酶插入这个肠袋时，在一些动物中它没有毒性且没有抗原性，而经腹腔注射的酶则明显具有抗原性。然而，通过维生素血浆浓度的升高并不能检测到这个人工器官合成了抗坏血酸。
