Costa R, Nigro L, Danieli G A
Biochem Genet. 1983 Feb;21(1-2):191-7. doi: 10.1007/BF02395403.
The biochemical properties of three allozymes coded by the Est-6 locus, two common forms (EST-6S and EST-6F) and one rare form (EST-6VF), were studied. The results show the existence of differences in isoelectric point, activity, activation energy, Km, and temperature coefficient among the three variants, especially between the two common forms and the one rare form. The specific activity of the rare enzymatic variant seems to be less affected by temperature variation. The possible significance of these findings in relation to the mechanism of reproduction is briefly discussed.
研究了由Est-6基因座编码的三种同工酶的生化特性，即两种常见形式（EST-6S和EST-6F）和一种罕见形式（EST-6VF）。结果表明，这三种变体在等电点、活性、活化能、米氏常数和温度系数方面存在差异，尤其是两种常见形式与一种罕见形式之间。罕见酶变体的比活性似乎受温度变化的影响较小。简要讨论了这些发现与繁殖机制相关的可能意义。
