促甲状腺激素释放激素（TRH）对体外培养的大鼠垂体前叶细胞释放黄体生成素（LH）的刺激作用。

Stimulatory effect of thyrotrophin-releasing hormone (TRH) on the release of luteinizing hormone (LH) from rat anterior pituitary cells in vitro.

作者信息

Fujihara N, Shiino M

出版信息

Can J Physiol Pharmacol. 1983 Feb;61(2):186-9. doi: 10.1139/y83-029.

Abstract

The effect of thyrotrophin-releasing hormone (TRH, 10(-7) M) on luteinizing hormone (LH) release from rat anterior pituitary cells was examined using organ and primary cell culture. The addition of TRH to the culture medium resulted in a slightly enhanced release of LH from the cultured pituitary tissues. However, the amount of LH release stimulated by TRH was not greater than that produced by luteinizing hormone-releasing hormone (LH-RH, 10(-7) M). Actinomycin D (2 X 10(-5) M) and cycloheximide (10(-4) M) had an inhibitory effect on the action of TRH on LH release. The inability of TRH to elicit gonadotrophin release from the anterior pituitary glands in vivo may partly be due to physiological inhibition of its action by other hypothalamic factor(s).

摘要

利用器官和原代细胞培养，研究了促甲状腺激素释放激素（TRH，10⁻⁷ M）对大鼠垂体前叶细胞促黄体生成素（LH）释放的影响。向培养基中添加TRH导致培养的垂体组织中LH释放略有增加。然而，TRH刺激的LH释放量并不比促黄体生成素释放激素（LH-RH，10⁻⁷ M）产生的量大。放线菌素D（2×10⁻⁵ M）和环己酰亚胺（10⁻⁴ M）对TRH促进LH释放的作用有抑制作用。TRH在体内无法引起垂体前叶释放促性腺激素，部分原因可能是其他下丘脑因子对其作用的生理抑制。