Matsubara T, Hirohata K
Exp Cell Biol. 1983;51(2):77-82. doi: 10.1159/000163177.
When macrophages were stimulated by concanavalin A (50 micrograms/ml) in the presence of 32Pi and 3H-arachidonic acid (AA), rapid incorporation of these radiolabeled substances into phosphatidylinositol (PI) and phosphatidic acid (PA) were induced within 10 min. The pool of PI and PA incorporating 3H-AA was the same as that of PI and PA which incorporated 32P. However, significant increase in incorporation of 32P and 3H-AA did not occur in phosphatidylcholine, phosphatidylethanolamine or phosphatidylserine within this time interval as compared with control levels. These results indicate that PI turnover might play a role in the initiation of prostaglandin biosynthesis in the early stages of macrophage activation induced by concanavalin A.
当巨噬细胞在32Pi和3H-花生四烯酸（AA）存在的情况下受到伴刀豆球蛋白A（50微克/毫升）刺激时，在10分钟内可诱导这些放射性标记物质迅速掺入磷脂酰肌醇（PI）和磷脂酸（PA）中。掺入3H-AA的PI和PA池与掺入32P的PI和PA池相同。然而，与对照水平相比，在此时间间隔内，磷脂酰胆碱、磷脂酰乙醇胺或磷脂酰丝氨酸中32P和3H-AA的掺入量并未显著增加。这些结果表明，PI周转可能在伴刀豆球蛋白A诱导的巨噬细胞激活早期阶段前列腺素生物合成的起始过程中发挥作用。
