Bercu B B, Lee B C, Spiliotis B E, Pineda J L, Denman D W, Hoffman H J, Brown T J
J Clin Endocrinol Metab. 1983 Jun;56(6):1227-35. doi: 10.1210/jcem-56-6-1227.
Pulsatile secretion of serum gonadotropins was studied in 16 castrated monkeys from 4 weeks of age through adult life. Animals were castrated at various ages from birth through adult life. Although some studies of the gonadotropin-secretory patterns were longitudinal in nature, most comparisons were cross-sectional. On the basis of our observations, we have arbitrarily grouped the animals into 4 developmental ages: postnatal (less than 7 months), prepubertal or juvenile (7-27 months), pubertal (28-59 months), and adult (greater than or equal to 60 months). In carrying out these studies, blood was withdrawn at 15-min intervals over 24 h without anesthesia using a mobile vest and tether assembly to support an indwelling catheter. GnRH challenge tests were done on 1 or more occasions on all animals. Plasma samples were analyzed for concentrations of FSH and LH by established RIAs and an in vitro bioassay for LH. During the frequent sampling period (24 h for all except postnatal animals), the amplitude of gonadotropin pulses was greatest in adult animals followed by postnatal and pubertal monkeys. During pubertal development, there was a marked increase in the magnitude of gonadotropin pulses, and remarkedly, there was a substantial increase in the LH bioassay: RIA (greater than 5:1) by adult life. GnRH challenge tests of gonadotropins correlated with these observations. Time series analysis was applied to the data for objective statistical characterization of cyclic patterns. Our findings can be summarized: 1) during pubertal maturation there is a change in amplitude but not frequency of gonadotropin pulses, 2) pubertal development of the hypothalamic-pituitary axis advances in the absence of gonadal feedback, and 3) there is a significant increase in the LH bioassay: RIA during pubertal development. We conclude that the castrate monkey is a valuable adjunct to direct clinical investigations of the mechanisms controlling human sexual development.
对16只从4周龄到成年期的去势猴子的血清促性腺激素脉冲分泌情况进行了研究。这些动物在从出生到成年期的不同年龄被去势。虽然一些关于促性腺激素分泌模式的研究本质上是纵向的，但大多数比较是横断面的。根据我们的观察，我们随意将这些动物分为4个发育年龄组：出生后（小于7个月）、青春期前或幼年（7 - 27个月）、青春期（28 - 59个月）和成年（大于或等于60个月）。在进行这些研究时，使用可移动背心和系绳组件支撑留置导管，在无麻醉的情况下，每隔15分钟采血一次，持续24小时。对所有动物进行了1次或多次GnRH激发试验。通过既定的放射免疫测定法和LH的体外生物测定法分析血浆样本中FSH和LH的浓度。在频繁采样期间（除出生后动物外均为24小时），成年动物促性腺激素脉冲的幅度最大，其次是出生后和青春期的猴子。在青春期发育过程中，促性腺激素脉冲的幅度显著增加，而且值得注意的是，到成年期LH生物测定值与放射免疫测定值的比值大幅增加（大于5:1）。促性腺激素的GnRH激发试验与这些观察结果相关。对数据应用时间序列分析以客观统计描述周期性模式。我们的发现可总结如下：1）在青春期成熟过程中，促性腺激素脉冲的幅度发生变化，但频率不变；2）下丘脑 - 垂体轴的青春期发育在无性腺反馈的情况下仍会推进；3）在青春期发育过程中，LH生物测定值与放射免疫测定值的比值显著增加。我们得出结论，去势猴子是直接临床研究控制人类性发育机制的有价值辅助手段。
