Kumar A A, Kempton R J, Anstead G M, Price E M, Freisheim J H
Anal Biochem. 1983 Jan;128(1):191-5. doi: 10.1016/0003-2697(83)90362-7.
A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.
已开发出一种利用反相半制备高效液相色谱柱和由三氟乙酸与1-丙醇组成的二元溶剂系统的方法，用于甲氨蝶呤四种新合成类似物的半制备规模纯化及分析鉴定。按所述方法以毫克量纯化了含有赖氨酸或鸟氨酸残基取代末端谷氨酸残基的甲氨蝶呤类似物及其各自的丹磺酰衍生物。
