Kaufmann M, Kubli F
Fortschr Med. 1978 Apr 20;96(15):823-6.
Using a short term incubation cell culture method with incorporation of radioisotopes the effect of cyclophosphamide and adriamycin to carcinoma cells and at the same time to bone marrow cells of the same patients has been evaluated in vitro. Instead of cyclophosphamide, ineffective in vitro, 4-Hydroperoxycyclophosphamide was tested. In case of the tumor cells innate as well as drug-induced resistances are obtained in vitro. Different resistance behavior of tumor and bone marrow cells to cyclophosphamide and adriamycin may indicate therapeutic results to be expected in vivo. Simultaneous in vitro resistance testing of tumor and normal cells offers the possibility of better characterization of tumor and host.
采用短期培养细胞法并结合放射性同位素，在体外评估了环磷酰胺和阿霉素对同一患者癌细胞以及同时对骨髓细胞的影响。用在体外无效的4-氢过氧环磷酰胺替代环磷酰胺进行了测试。在肿瘤细胞中，体外获得了天然抗性以及药物诱导的抗性。肿瘤细胞和骨髓细胞对环磷酰胺和阿霉素的不同抗性行为可能预示体内预期的治疗结果。肿瘤细胞和正常细胞的同步体外抗性测试为更好地表征肿瘤和宿主提供了可能性。
