Maeda T, Balakrishnan K, Mehdi S Q
Biochim Biophys Acta. 1983 May 26;731(1):115-20. doi: 10.1016/0005-2736(83)90404-2.
A simple and rapid method for preparing plasma membranes from isolated cells or tissues is described. The membranes were characterised (a) biochemically by an analysis of specific marker enzymes, (b) by quantitation of cell surface receptors, and (c) immunologically by their ability to elicit specific allogeneic responses from cytotoxic T cells in secondary in vitro stimulations. Based on both biochemical and immunologic criteria, plasma membranes prepared by the method described here are of equal or greater 'purity' compared to those prepared by two other methods that are most widely used to date and the yields are several-fold higher.
本文描述了一种从分离的细胞或组织中制备质膜的简单快速方法。通过以下方式对这些膜进行了表征：(a) 对特定标记酶进行分析以进行生化表征，(b) 对细胞表面受体进行定量分析，以及 (c) 通过其在二次体外刺激中引发细胞毒性T细胞特异性同种异体反应的能力进行免疫学表征。基于生化和免疫学标准，与目前最广泛使用的其他两种方法制备的质膜相比，本文所述方法制备的质膜具有同等或更高的“纯度”，且产量高出数倍。
