Yue T L, Varma D R, Powell W S
Biochim Biophys Acta. 1983 May 16;751(3):332-9. doi: 10.1016/0005-2760(83)90291-6.
The effects of protein deficiency on the biosynthesis of metabolites of arachidonic acid by rat pleural polymorphonuclear leukocytes stimulated with calcium ionophore were investigated. The major products of metabolism by lipoxygenase in these cells were leukotriene B4 and 5-hydroxy-6,8,11,14-eicosatetraenoic acid, whereas the major cyclooxygenase products were thromboxane B2 and 12-hydroxy-5,8,10-heptadecatrienoic acid. At high substrate concentrations (100 microM), the formation of all products by polymorphonuclear leukocytes was lower for protein-deficient rats than for controls. Similar results were obtained when products synthesized from endogenous substrate were measured, except that there was no change in the amount of 5-hydroxy-6,8,11,14-eicosatetraenoic acid formed. The biosynthesis of prostaglandins E2 and F2 alpha by homogenates of rat kidney medulla was reduced as a result of protein deficiency. Acetylsalicylic acid inhibited the formation of cyclooxygenase products and stimulated the formation of lipoxygenase products by polymorphonuclear leukocytes. Protein deficiency did not alter the effects of acetylsalicylic acid on the biosynthesis of these products, although at any given concentration the amounts of products formed were less with protein-deficient rats than with rats fed control diets.
研究了蛋白质缺乏对钙离子载体刺激的大鼠胸膜多形核白细胞花生四烯酸代谢产物生物合成的影响。这些细胞中脂氧合酶的主要代谢产物是白三烯B4和5-羟基-6,8,11,14-二十碳四烯酸，而环氧化酶的主要产物是血栓素B2和12-羟基-5,8,10-十七碳三烯酸。在高底物浓度（100 microM）下，蛋白质缺乏大鼠的多形核白细胞形成的所有产物均低于对照组。当测量由内源性底物合成的产物时，得到了类似的结果，只是5-羟基-6,8,11,14-二十碳四烯酸的形成量没有变化。蛋白质缺乏导致大鼠肾髓质匀浆中前列腺素E2和F2α的生物合成减少。乙酰水杨酸抑制多形核白细胞环氧化酶产物的形成并刺激脂氧合酶产物的形成。蛋白质缺乏并未改变乙酰水杨酸对这些产物生物合成的影响，尽管在任何给定浓度下，蛋白质缺乏大鼠形成的产物量都比喂食对照饮食的大鼠少。
