苯丙氨酰-tRNA合成酶和苏氨酰-tRNA合成酶过量产生对大肠杆菌细胞中tRNAPhe和tRNAThr浓度的影响。
Effect of the overproduction of phenylalanyl- and threonyl-tRNA synthetases on tRNAPhe and tRNAThr concentrations in E. coli cells.
作者信息
Fayat G, Fromant M, Kalogerakos T, Blanquet S
出版信息
Biochimie. 1983 Mar;65(3):221-5. doi: 10.1016/s0300-9084(83)80087-x.
Transformation of an E. coli strain with a recombinant plasmid DNA (pB1) encoding the genes for phenylalanyl- and threonyl-tRNA synthetases causes overproduction of these enzymes by about 100- and 5-fold, respectively. A possible effect of the overproduction of the two aminoacyl-tRNA synthetases on intracellular cognate tRNA levels has been searched for by comparing tRNAThr and tRNAPhe aminoacylation capacities in the RNA extracts from strains carrying pB1 or pBR322 plasmid DNA. The answer is that the levels of these tRNAs are not changed by selective increase of the cognate synthetases.
用编码苯丙氨酰 - tRNA合成酶和苏氨酰 - tRNA合成酶基因的重组质粒DNA（pB1）转化大肠杆菌菌株，会使这些酶的过量表达分别提高约100倍和5倍。通过比较携带pB1或pBR322质粒DNA的菌株的RNA提取物中tRNAThr和tRNAPhe的氨酰化能力，研究了两种氨酰 - tRNA合成酶过量表达对细胞内同源tRNA水平的可能影响。结果表明，同源合成酶的选择性增加并不会改变这些tRNA的水平。
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