Miyazaki M, Makowka L, Falk R E, Falk J A, Inaba T
Can J Surg. 1983 May;26(3):224-8.
A model for the in-vivo perfusion of the isolated rat liver with either cytotoxic agents or hyperthermic saline, administered through either the hepatic artery or the portal vein, is described. Rats were able to survive following perfusion with a dose of up to 0.25 g/kg of 5-fluorouracil and 2.0 to 2.5 mg/kg of mitomycin-C by both routes. Regional hyperthermic perfusion of the liver produced temperature and time-dependent hepatotoxic effects. A marked elevation of serum glutamic oxaloacetic and glutamic pyruvic transaminase levels was noted 24 hours after perfusion, but was reversible. Aminopyrine N-demethylase activity in both normal intact and regenerating rat liver was not affected; however, the activity in normal intact liver was markedly suppressed with hyperthermic perfusion at 43 degrees C only. Incorporation of tritiated thymidine into hepatic deoxyribonucleic acid 24 hours after partial hepatectomy was severely inhibited by prior hepatic perfusion with either cytotoxic drugs or hyperthermic saline. This in-vivo isolated perfusion technique could be used to assess the effect of thermochemotherapy on tumour growth in the liver.
本文描述了一种用于对离体大鼠肝脏进行体内灌注的模型，灌注的物质可以是细胞毒性药物或热盐水，通过肝动脉或门静脉给药。通过这两种途径，大鼠在灌注高达0.25 g/kg的5-氟尿嘧啶和2.0至2.5 mg/kg的丝裂霉素-C后仍能存活。肝脏区域热灌注产生了温度和时间依赖性的肝毒性作用。灌注后24小时，血清谷草转氨酶和谷丙转氨酶水平显著升高，但可逆转。正常完整和再生大鼠肝脏中的氨基比林N-脱甲基酶活性均未受影响；然而，仅在43℃进行热灌注时，正常完整肝脏中的该活性受到显著抑制。部分肝切除术后24小时，将氚标记的胸腺嘧啶掺入肝脱氧核糖核酸的过程受到先前用细胞毒性药物或热盐水进行肝灌注的严重抑制。这种体内离体灌注技术可用于评估热化疗对肝脏肿瘤生长的影响。
