Lönn U, Lönn S
Carcinogenesis. 1983;4(5):583-6. doi: 10.1093/carcin/4.5.583.
Ascorbate-Cu2+ shows considerable cytotoxicity for human melanoma cells at a dose which has very little effect on human fibroblasts. Ascorbate itself inhibits DNA synthesis in melanoma cells but does not fragment the parental DNA. However, the combined action of ascorbate-Cu2+ generates fragmentation of the parental DNA due to the induction of alkali-labile bonds in the DNA. In contrast, if DNA polymerase alpha is inhibited by aphidicolin prior to treatment with ascorbate-Cu2+ one cannot detect the fragmentation of the DNA. The generated fragments show a discrete appearance in agarose gel electrophoresis with a single-stranded size of approximately 5 kb. When fibroblasts were analyzed using the same experimental protocol it was not possible to detect the fragmentation of the DNA.
抗坏血酸盐 - Cu²⁺ 对人黑色素瘤细胞具有显著的细胞毒性，而该剂量对人成纤维细胞几乎没有影响。抗坏血酸盐本身可抑制黑色素瘤细胞中的DNA合成，但不会使亲本DNA片段化。然而，抗坏血酸盐 - Cu²⁺ 的联合作用会导致亲本DNA片段化，这是由于DNA中诱导产生了碱不稳定键。相反，如果在用抗坏血酸盐 - Cu²⁺ 处理之前用阿非迪霉素抑制DNA聚合酶α，则无法检测到DNA的片段化。所产生的片段在琼脂糖凝胶电泳中呈现离散的外观，单链大小约为5 kb。当使用相同的实验方案分析成纤维细胞时，无法检测到DNA的片段化。
