Sindelar W F, Morrow B M, Travis E L, Tepper J, Merkel A B, Kranda K, Terrill R
Int J Radiat Oncol Biol Phys. 1983 Apr;9(4):523-32. doi: 10.1016/0360-3016(83)90071-8.
Adult dogs were subjected to laparotomy and intraoperative electron irradiation after division and reanastomosis of aorta or after construction of a blind loop of small intestine having a transverse suture line and an end-to-side anastomosis. Dogs received intraoperative irradiation of both intact and anastomosed aorta or intestine in doses of 0, 2000, 3000, or 4500 rad. Animals were sacrificed at seven days or three months following treatment. At 24 hours prior to sacrifice, dogs received 5 mCi tritiated thymidine intravenously. Irradiated and non-irradiated segments of aorta and small intestine, including intact and anastomotic regions, were analyzed for tritiated thymidine incorporation and were subjected to autoradiography. Incorporation studies showed diminution in tritiated thymidine uptake by irradiated portions of aorta and small intestine, in both intact and anastomotic regions. Autoradiograms revealed that irradiated areas of intact or anastomotic aorta or intestine had diminished labeling of stromal cells, suggesting a lowered cell proliferative capacity of irradiated tissue compared to non-irradiated portions. Inflammatory cells showed similar labeling indices in irradiated and non-irradiated tissues, both intact and surgically-manipulated, suggesting that irradiation does not significantly affect a subsequent local inflammatory response. Radiation-induced decreases in tritiated thymidine incorporation in irradiated aorta and small intestine were generally more marked at seven days than at three months following irradiation, suggesting that radiation-induced depression of cell turnover rates decreases with time. The presence of tritiated thymidine uptake after irradiation demonstrates the ability of intact and surgically-manipulated aorta and intestine to recover from radiation-induced damage.
成年犬在主动脉分离并重新吻合后，或在构建带有横向缝合线和端侧吻合术的小肠盲袢后，接受剖腹术和术中电子照射。犬接受剂量为0、2000、3000或4500拉德的完整和吻合后主动脉或小肠的术中照射。在治疗后7天或3个月处死动物。在处死前24小时，犬静脉注射5毫居里氚标记胸腺嘧啶核苷。对主动脉和小肠的照射和未照射段，包括完整和吻合区域，进行氚标记胸腺嘧啶核苷掺入分析并进行放射自显影。掺入研究表明，在完整和吻合区域，主动脉和小肠照射部分的氚标记胸腺嘧啶核苷摄取减少。放射自显影显示，完整或吻合主动脉或小肠的照射区域间质细胞标记减少，表明与未照射部分相比，照射组织的细胞增殖能力降低。炎症细胞在照射和未照射组织（包括完整和手术操作的组织）中的标记指数相似，表明照射不会显著影响随后的局部炎症反应。照射后主动脉和小肠中氚标记胸腺嘧啶核苷掺入的辐射诱导减少在照射后7天通常比3个月时更明显，表明辐射诱导的细胞更新率抑制随时间降低。照射后氚标记胸腺嘧啶核苷摄取的存在证明了完整和手术操作的主动脉和小肠从辐射诱导损伤中恢复的能力。
