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克隆的荚膜红假单胞菌光合作用基因与其他光合细菌DNA的杂交。

Hybridization of cloned Rhodopseudomonas capsulata photosynthesis genes with DNA from other photosynthetic bacteria.

作者信息

Beatty J T, Cohen S N

出版信息

J Bacteriol. 1983 Jun;154(3):1440-5. doi: 10.1128/jb.154.3.1440-1445.1983.

Abstract

The homology of Rhodopseudomonas capsulata DNA segments carrying photosynthesis genes with sequences present in total DNA from certain other photosynthetic and non-photosynthetic bacterial species was determined by hybridization. R. capsulata DNA fragments that carry loci for production of peptide components of the photosynthetic reaction center and light-harvesting I antenna complex were found to hybridize to DNA from some photosynthetic species. However, fragments that carry carotenoid or bacteriochlorophyll biosynthesis genes showed either weak or undetectable heterospecific hybridization under the conditions employed.

摘要

通过杂交测定了携带光合作用基因的荚膜红假单胞菌DNA片段与某些其他光合和非光合细菌物种的总DNA中存在的序列的同源性。发现携带光合反应中心和光捕获I天线复合体肽成分产生位点的荚膜红假单胞菌DNA片段可与一些光合物种的DNA杂交。然而,在所用条件下,携带类胡萝卜素或细菌叶绿素生物合成基因的片段显示出弱的或无法检测到的异源特异性杂交。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3a0/217621/279968989ca6/jbacter00247-0421-a.jpg

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