Tsuji T, Tsunehisa S, Watanabe Y, Yamamoto K, Tohyama H, Osawa T
J Biol Chem. 1983 May 25;258(10):6335-9.
Glycocalicin, a predominant glycoprotein on the human platelet surface, has been purified from a platelet suspension by sonication, ammonium sulfate precipitation and acid treatment followed by chromatography on columns of wheat germ agglutinin-Sepharose and Sephacryl S-300. Ser/Thr-linked (O-linked) oligosaccharides were released by alkaline borohydride treatment, and fractionated by high performance liquid chromatography with an anion-exchange resin. The structure of a major oligosaccharide alditol separated by high performance liquid chromatography was investigated by a combination of compositional analyses, methylation and glycosidase treatments, and proposed to be a hexasaccharide alditol, NeuAc alpha 2-3Gal beta 1-4GlcNAc beta 1-6(NeuAc alpha 2-3Gal beta 1-3) N-acetylgalactosaminitol. We also found some sugar units which appeared to be intermediates in the biosynthetic pathway of the major hexasaccharide.
糖钙蛋白是人类血小板表面的一种主要糖蛋白，通过超声处理、硫酸铵沉淀、酸处理，随后在麦胚凝集素-琼脂糖柱和Sephacryl S-300柱上进行色谱分离，从血小板悬液中纯化得到。通过碱性硼氢化钠处理释放出丝氨酸/苏氨酸连接（O连接）的寡糖，并用阴离子交换树脂通过高效液相色谱进行分级分离。通过组成分析、甲基化和糖苷酶处理相结合的方法研究了通过高效液相色谱分离得到的一种主要寡糖醇的结构，推测其为一种六糖糖醇，即NeuAcα2-3Galβ1-4GlcNAcβ1-6(NeuAcα2-3Galβ1-3)N-乙酰半乳糖胺醇。我们还发现了一些似乎是主要六糖生物合成途径中间产物的糖单元。
