牛精液冷冻干燥过程中精液蛋白质的变化

Alteration of seminal proteins during freeze-drying of bovine semen.

作者信息

Jeyendran R S, Hunter A G, Graham E F

出版信息

J Dairy Sci. 1983 Apr;66(4):887-91. doi: 10.3168/jds.S0022-0302(83)81872-4.

Abstract

Bovine semen in TEST-yolk extender was frozen, freeze-dried to 50, 25, 12, 6, and less than 2% residual moisture, and stored at -196 degrees C. The freeze-dried semen was rehydrated, sampled for protein analysis, and used to inseminate cattle. Agar gel electrophoresis revealed no moisture was reduced to less than 6%. At that point, the percent of cationic proteins and neural proteins decreased with a concurrent increase in anionic migrating proteins. Immunodiffusion data with antisera against spermatozoa and seminal plasma revealed no difference in formation of precipitin lines if residual moisture was at least 6%. However, semen freeze-dried to 2% residual moisture was modified antigenically as certain precipitin lines were lost, new lines appeared, and concentration of other seminal antigens decreased. The absence of fertility with semen freeze-dried to 2% residual moisture is hypothesized to 2% residual moisture is hypothesized to be from alteration of the tertiary structure of certain essential seminal proteins.

摘要

将保存在TEST-蛋黄稀释液中的牛精液冷冻，然后冻干至残留水分分别为50%、25%、12%、6%及低于2%，并储存在-196℃。将冻干的精液复水，取样进行蛋白质分析，并用于给母牛输精。琼脂凝胶电泳显示，当残留水分降至低于6%时，阳离子蛋白和神经蛋白的百分比下降，同时阴离子迁移蛋白增加。用抗精子和精浆抗血清进行免疫扩散数据显示，如果残留水分至少为6%，沉淀线的形成没有差异。然而，冻干至残留水分2%的精液在抗原性上发生了改变，因为某些沉淀线消失，出现了新的沉淀线，其他精浆抗原的浓度也降低了。冻干至残留水分2%的精液不育被认为是某些必需精浆蛋白的三级结构发生改变所致。