Cohen B B, Deane D L, Moxley M, Steel C M
J Immunol Methods. 1983 Jun 24;61(1):91-7. doi: 10.1016/0022-1759(83)90012-1.
Ia antigens were shown to be present in the cell almost exclusively as mature alpha beta dimers which split into separate alpha and beta chains after boiling in SDS. In contrast metabolically labelling the cells with [35S]methionine resulted in only free alpha and beta chains being labelled. It is concluded that this widely used type of labelling, although useful for studying intermediate synthesis, should not be used for labelling mature cell surface molecules.
Ia抗原几乎仅以成熟的αβ二聚体形式存在于细胞中，这些二聚体在SDS中煮沸后会分裂成单独的α链和β链。相比之下，用[35S]甲硫氨酸对细胞进行代谢标记，结果只有游离的α链和β链被标记。得出的结论是，这种广泛使用的标记类型虽然对研究中间合成有用，但不应被用于标记成熟的细胞表面分子。
