de Gier J J, t Hart B J, Wilting J
Methods Find Exp Clin Pharmacol. 1983;5(1):49-53.
Serum levels of free diazepam as determined with equilibrium dialysis depend on the composition of the dialyzing buffer with respect to Ca2+ and Cl-. Physiological concentrations of these ions double the obtained free serum levels, in line with data of diazepam containing albumin solutions. The diazepam binding capacity of serum was found to be higher than that of albumin alone, suggesting that albumin is probably not the only diazepam binding protein in serum. Evidence was also found that the diazepam binding to these other serum proteins is dependent on the presence of Ca2+ and Cl-.
通过平衡透析测定的血清中游离地西泮水平取决于透析缓冲液中钙离子（Ca2+）和氯离子（Cl-）的组成。这些离子的生理浓度会使测得的游离血清水平翻倍，这与含白蛋白的地西泮溶液的数据一致。研究发现血清的地西泮结合能力高于单独白蛋白的结合能力，这表明白蛋白可能不是血清中唯一与地西泮结合的蛋白质。还发现有证据表明地西泮与这些其他血清蛋白的结合取决于钙离子和氯离子的存在。
