Vijayan E, Samson W K, McCann S M
Proc Soc Exp Biol Med. 1983 May;173(1):153-8. doi: 10.3181/00379727-173-41624.
Arginine vasotocin was injected into the third ventricle or intravenously in conscious, ovariectomized rats and its effect on gonadotropin and prolactin release evaluated. The peptide lowered plasma levels of both LH and prolactin in doses of 40 or 100 ng given intraventricularly. The higher dose was slightly more effective than the lower dose. Intravenous injection of a 1-microgram dose of vasotocin failed to alter plasma LH in the ovariectomized animals; however, a 5-micrograms dose induced a slight depression apparent at only 60 min following injection. Intravenous injection of 1 microgram produced a significant lowering of plasma prolactin, whereas a dramatic lowering followed the injection of the higher dose. Plasma FSH was unaffected in these experiments. Incubation of dispersed anterior pituitary cells from ovariectomized rats with various doses of vasotocin revealed no effect of the peptide on the release of FSH, LH, or prolactin. It also did not alter the response to LHRH, but it partially blocked the action of dopamine to inhibit prolactin release. The data indicate that quite low doses of arginine vasotocin act within the brain to inhibit LH and prolactin secretion in ovariectomized, conscious animals.
将精氨酸加压素注入清醒的去卵巢大鼠的第三脑室或静脉内，并评估其对促性腺激素和催乳素释放的影响。脑室注射40或100纳克剂量的该肽可降低血浆中促黄体生成素（LH）和催乳素的水平。较高剂量比较低剂量稍有效。静脉注射1微克剂量的加压素未能改变去卵巢动物的血浆LH水平；然而，5微克剂量在注射后仅60分钟时引起轻微下降。静脉注射1微克可显著降低血浆催乳素水平，而注射较高剂量后则显著降低。在这些实验中，血浆促卵泡激素（FSH）未受影响。用不同剂量的加压素孵育去卵巢大鼠分散的垂体前叶细胞，结果显示该肽对FSH、LH或催乳素的释放没有影响。它也没有改变对促性腺激素释放激素（LHRH）的反应，但部分阻断了多巴胺抑制催乳素释放的作用。数据表明，相当低剂量的精氨酸加压素在脑内起作用，抑制去卵巢清醒动物的LH和催乳素分泌。
