甲状腺激素与肝脏UDP-葡萄糖醛酸基转移酶活性：大鼠和小鼠中的相反作用

Thyroid hormone and hepatic UDP-glucuronosyl transferase activity: contrary effects in rat and mouse.

作者信息

Moscioni A D, Gartner L M

出版信息

Res Commun Chem Pathol Pharmacol. 1983 Mar;39(3):445-62.

Abstract

The administration of tri-iodothyronine (T3) to female Sprague-Dawley (SD) rats lowered the basal activity of uridine diphosphoglucuronate glucuronosyl transferase (UDPGT:EC 2.4.1.17) for bilirubin (Bili) by 75 percent, but dramatically increased the level of UDP-glucuronosyl transferase for p-nitrophenol (UDPGT-PNP) by more than 100 percent. Similar experiments performed in four strains of female mice produced results contrary to those observed in the rat. T3 administered to C57BL/6J, DBA/2J, Swiss-Webster (SW) and CD-1 mice significantly increased hepatic UDPGT-Bili by 28, 69, 79 and 94%, respectively. The increase of UDPGT-Bili in the selected strains was observed to be inversely and linearly proportional to the basal level of enzyme measured in each strain (r = -0.99215; p less than 0.001). Only in the DBA/2J strain was UDPGT-PNP significantly diminished to 66% of control levels, producing a result exactly opposite to that observed in the SD rat; in the other three strains, UDPGT-PNP was not significantly altered.

摘要

给雌性斯普拉格-道利（SD）大鼠注射三碘甲状腺原氨酸（T3），可使胆红素（Bili）的尿苷二磷酸葡萄糖醛酸葡萄糖醛酸基转移酶（UDPGT：EC 2.4.1.17）的基础活性降低75%，但对硝基苯酚的UDP-葡萄糖醛酸基转移酶（UDPGT-PNP）水平却显著升高超过100%。在四种雌性小鼠品系中进行的类似实验产生了与在大鼠中观察到的结果相反的结果。给C57BL/6J、DBA/2J、瑞士-韦伯斯特（SW）和CD-1小鼠注射T3后，肝脏UDPGT-Bili分别显著增加了28%、69%、79%和94%。在所选品系中观察到的UDPGT-Bili的增加与每个品系中测得的酶的基础水平呈反比且线性相关（r = -0.99215；p < 0.001）。仅在DBA/2J品系中，UDPGT-PNP显著降低至对照水平的66%，产生了与在SD大鼠中观察到的结果完全相反的结果；在其他三个品系中，UDPGT-PNP没有显著改变。