Ebersole J L, Molinari J A
Immunology. 1978 Mar;34(3):379-87.
Topical oral immunization of axenic mice with non-replicating Staphylococcus aureus resulted in the production of both serum and salivary agglutinins. A latent period of approximately 5 days was noted before salivary antibodies were detected against both a carbohydrate (ChoA) and a protein (PrA) antigen isolated from this micro-organism. The local response to ChoA exhibited a linear increase until day 9 when the reciprocal titres reached a plateau [32 (16--64)]. Salivary antibodies to PrA peaked by day 13 at a mean reciprocal titre of 60 (32--128). The agglutinin response in saliva was found to be initially IgG; however, by day 9 of a 14-day-immunization regimen, IgA became the predominant class of exocrine anti-S. aureus antibodies. The serum agglutinin response followed that found in saliva by approximately 2--4 days. By day 14, all sera (6/6) contained PrA agglutinins, while 4/6 sera agglutinated sheep erythrocytes coated with ChoA. Serum antibodies to ChoA were exclusively IgM, in contrast to IgM and IgA agglutinins elicited by PrA. Absorption studies provided evidence of a specific local and systemic immune response to both ChoA and PrA antigens of perorally administered S. aureus.
用非复制型金黄色葡萄球菌对无菌小鼠进行局部口服免疫，可导致血清和唾液凝集素的产生。在检测到针对从该微生物分离的碳水化合物（ChoA）和蛋白质（PrA）抗原的唾液抗体之前，观察到约5天的潜伏期。对ChoA的局部反应呈线性增加，直到第9天，其倒数滴度达到平台期[32（16 - 64）]。对PrA的唾液抗体在第13天达到峰值，平均倒数滴度为60（32 - 128）。发现唾液中的凝集素反应最初是IgG；然而，在14天免疫方案的第9天，IgA成为外分泌抗金黄色葡萄球菌抗体的主要类别。血清凝集素反应比唾液中的反应晚约2 - 4天出现。到第14天，所有血清（6/6）都含有PrA凝集素，而4/6的血清能凝集包被有ChoA的绵羊红细胞。与PrA引发的IgM和IgA凝集素不同，针对ChoA的血清抗体仅为IgM。吸收研究提供了证据，表明对口服给予的金黄色葡萄球菌的ChoA和PrA抗原存在特异性局部和全身免疫反应。
