Schwarze W, Bernhardt R, Jänig G R, Ruckpaul K
Biochem Biophys Res Commun. 1983 May 31;113(1):353-60. doi: 10.1016/0006-291x(83)90473-4.
The distance between the heme iron and the N-terminus of cytochrome P-450 LM2 was determined by fluorescence energy transfer measurements. Fluorescein isothiocyanate which was covalently bound to the N-terminal methionine was used as donor chromophor. The Ro value between fluorescein isothiocyanate and the heme was calculated to be 3.98 nm. The distance between the nitrogen of the N-terminal methionine and the heme was estimated with 2.84 +/- 0.23 nm excluding most likely the N-terminal amino acid of cytochrome P-450 LM2 to participate in the electron transfer to the heme iron. A cytochrome P-450 LM2 membrane model is proposed.
通过荧光能量转移测量确定了细胞色素P-450 LM2的血红素铁与N端之间的距离。将共价结合到N端甲硫氨酸上的异硫氰酸荧光素用作供体发色团。计算出异硫氰酸荧光素与血红素之间的Ro值为3.98 nm。估计N端甲硫氨酸的氮与血红素之间的距离为2.84±0.23 nm，最有可能排除细胞色素P-450 LM2的N端氨基酸参与向血红素铁的电子转移。提出了一种细胞色素P-450 LM2膜模型。
