从卵巢癌患者腹水中亲和层析分离肿瘤相关抗原。

Affinity chromatography separation of tumor associated antigens from ascitic fluid of ovarian cancer patients.

作者信息

Hill R, Daunter B, Silburn P A, Khoo S K, Mackay E V

出版信息

Gynecol Oncol. 1983 Jun;15(3):428-33. doi: 10.1016/0090-8258(83)90062-8.

Abstract

Tumor associated immunoglobulins previously isolated from ovarian cancer ascitic fluid have been used in an affinity chromatography step to purify putative tumor associated antigens from a perchloric acid extract of ascitic fluid. Examination of the affinity chromatography purified proteins revealed 4 bands on electrophoresis. Antisera raised to the affinity chromatography products have been examined by Ouchterlony diffusion as well as a solid-phase radioimmunoassay. Two of thirteen ovarian tumor extracts investigated reacted in Ouchterlony diffusion analysis, whereas all thirteen extracts gave positive results in the more sensitive solid-phase radioimmunoassay.

摘要

先前从卵巢癌腹水中分离出的肿瘤相关免疫球蛋白已用于亲和层析步骤，以从腹水的高氯酸提取物中纯化假定的肿瘤相关抗原。对亲和层析纯化的蛋白质进行电泳检测，发现有4条带。已通过双向免疫扩散以及固相放射免疫测定法检测了针对亲和层析产物产生的抗血清。在接受研究的13份卵巢肿瘤提取物中，有两份在双向免疫扩散分析中出现反应，而在更灵敏的固相放射免疫测定中，所有13份提取物均给出了阳性结果。