Ross J L, Loriaux D L, Cutler G B
J Clin Endocrinol Metab. 1983 Aug;57(2):288-93. doi: 10.1210/jcem-57-2-288.
Patients with gonadal dysgenesis have a marked increase in gonadotropin levels at the age when puberty normally occurs. To determine whether this increase results from a change in the frequency or the amplitude of gonadotropin pulses, we measured the 24-h profile of plasma LH and FSH by RIA in 31 patients with gonadal dysgenesis, aged 2-20 yr. Gonadotropin pulses were defined as a rise from nadir to peak that exceeded 3 times the intraassay coefficient of variation. This criterion, based on an empirical study of RIA noise, reduced the rate of false positive peaks to less than 3-4/24 h. Using this criterion, peak amplitude increased significantly at the time of puberty for both LH and FSH (P less than 0.01). The overall frequency of gonadotropin pulses (the sum of the FSH peaks plus the LH peaks that occurred without a concomitant FSH peak), however, did not differ among prepubertal (12.7 +/- 1.8 peaks/24 h), pubertal aged (14.3 +/- 2.3 peaks/24 h), and adult patients (14.7 +/- 0.9 peaks/24 h). Thus, the increase in gonadotropin concentration in pubertal aged patients with gonadal dysgenesis appears to result primarily from an increase in gonadotropin peak amplitude rather than an increase in peak frequency.
在正常青春期年龄时，性腺发育不全患者的促性腺激素水平显著升高。为了确定这种升高是由促性腺激素脉冲频率还是幅度的变化引起的，我们通过放射免疫分析法（RIA）测量了31例年龄在2至20岁的性腺发育不全患者血浆促黄体生成素（LH）和促卵泡生成素（FSH）的24小时变化曲线。促性腺激素脉冲被定义为从最低点到峰值的上升超过测定内变异系数的3倍。基于对RIA噪声的实证研究，该标准将假阳性峰值率降低至小于3 - 4次/24小时。使用该标准，LH和FSH在青春期时峰值幅度均显著增加（P小于0.01）。然而，促性腺激素脉冲的总体频率（FSH峰值加上不伴有FSH峰值的LH峰值之和）在青春期前患者（12.7±1.8次峰值/24小时）、青春期患者（14.3±2.3次峰值/24小时）和成年患者（14.7±0.9次峰值/24小时）之间并无差异。因此，青春期性腺发育不全患者促性腺激素浓度的升高似乎主要是由促性腺激素峰值幅度增加而非峰值频率增加所致。
