Kaneoka H, Perez-Rojas G, Sasasuki T, Benike C J, Engleman E G
J Immunol. 1983 Jul;131(1):158-64.
Anti-Leu-4 is a murine monoclonal antibody that defines a molecule of 20,000 to 25,000 daltons present on all mature T lymphocytes in man. When cultured in the presence of 10 to 1000 ng/ml anti-Leu-4, the T cells of most individuals proliferate with peak responses on the third day of culture. T cells of both helper and suppressor lineages proliferate, but only in the presence of monocytes. Approximately 40% of individuals tested responded weakly or not at all to anti-Leu-4, despite normal responses to other stimuli. The variation in responsiveness between individuals could not be explained by differences in Leu-4 antigen density on the surface of T cells, differences in the rate of Leu-4 antigen modulation, or structural differences in the Leu-4 molecule as defined by the method of two-dimensional polyacrylamide gel electrophoresis. In the presence of monocytes from high responders, the T cells from low responders proliferated vigorously to anti-Leu-4, whereas monocytes from low responders failed to support proliferation by high responder T cells. On the other hand, low responder monocytes did not prevent T cells from proliferating in the presence of high responder monocytes. These results suggest that the failure of some individuals to respond to anti-Leu-4 is due to the absence or dysfunction of an essential monocyte population.
抗Leu-4是一种鼠单克隆抗体，它可识别存在于人类所有成熟T淋巴细胞上的一种分子量为20,000至25,000道尔顿的分子。当在10至1000 ng/ml抗Leu-4存在的情况下进行培养时，大多数个体的T细胞会增殖，在培养的第三天出现增殖高峰。辅助性和抑制性谱系的T细胞都会增殖，但仅在单核细胞存在的情况下。尽管对其他刺激有正常反应，但约40%接受检测的个体对抗Leu-4反应微弱或根本无反应。个体之间反应性的差异无法通过T细胞表面Leu-4抗原密度的差异、Leu-4抗原调节速率的差异或二维聚丙烯酰胺凝胶电泳法所定义的Leu-4分子的结构差异来解释。在存在高反应者的单核细胞时，低反应者的T细胞对抗Leu-4会强烈增殖，而低反应者的单核细胞则无法支持高反应者T细胞的增殖。另一方面，低反应者的单核细胞不会阻止T细胞在高反应者单核细胞存在的情况下增殖。这些结果表明，一些个体对抗Leu-4无反应是由于缺乏必需的单核细胞群体或其功能障碍所致。
