Mitani H
Mutat Res. 1983 Feb;107(2):279-88. doi: 10.1016/0027-5107(83)90169-0.
GEM 199 cells derived from an erythrophoroma of goldfish (Carassius auratus), which had a high plating efficiency, were used to investigate the lethal and mutational effects of radiations (UV and gamma-rays) and chemicals (4NQO and MNNG). The cells were more resistant to gamma-rays than mammalian cells and CAF-MM1 cells derived from the normal fin tissue of goldfish. They were also more resistant to UV-irradiation than CAF-MM1 cells. Photoreactivation after UV-irradiation was present in GEM 199 cells for both survival and mutation. The initial shoulder of the survival curve of UV-irradiated cells was reduced greatly by caffeine, suggesting a high activity of the post-replication repair. The spontaneous mutation frequency to ouabain resistance was 1-5 X 10(-6) clones per viable cell. MNNG was effective in inducing ouabain-resistant mutation, while 4NQO and gamma-rays did not induce mutation.
源自金鱼（Carassius auratus）红斑瘤、具有高接种效率的GEM 199细胞，被用于研究辐射（紫外线和γ射线）及化学物质（4-硝基喹啉-N-氧化物和N-甲基-N'-硝基-N-亚硝基胍）的致死和诱变效应。这些细胞比哺乳动物细胞以及源自金鱼正常鳍组织的CAF-MM1细胞对γ射线更具抗性。它们对紫外线照射的抗性也比CAF-MM1细胞更强。紫外线照射后的光复活现象在GEM 199细胞的存活和突变方面均有体现。咖啡因可大幅降低紫外线照射细胞存活曲线的初始平台期，这表明复制后修复活性较高。对哇巴因抗性的自发突变频率为每存活细胞1 - 5×10⁻⁶个克隆。N-甲基-N'-硝基-N-亚硝基胍可有效诱导对哇巴因的抗性突变，而4-硝基喹啉-N-氧化物和γ射线则不会诱导突变。
