Prehm P
Biochem J. 1983 Apr 1;211(1):181-9. doi: 10.1042/bj2110181.
Differentiation of teratocarcinoma cells led to induction of hyaluronate synthesis. The synthase was recovered in the membrane fraction of cell lysates. Hyaluronate was synthesized at the membranes and was then released as a soluble product. The synthase could be stimulated by a variety of phosphate esters which prevented the degradation of the substrates UDP-GlcNAc and UDP-GlcA and the release of the growing hyaluronic acid chain from the membrane. Hyaluronidases or oligosaccharides derived from hyaluronate did not affect the synthesis. The chains grew at a rate of 60 repeating units/min. Continuous new chain initiation occurred during prolonged synthesis. Digestion of pulse-chase-labelled hyaluronate with beta-N-acetylglucosaminidase and beta-glucuronidase showed that the chains grew at the reducing end.
畸胎癌细胞的分化导致透明质酸合成的诱导。合酶在细胞裂解物的膜部分中回收。透明质酸在膜上合成,然后作为可溶性产物释放。合酶可被多种磷酸酯刺激,这些磷酸酯可防止底物UDP-GlcNAc和UDP-GlcA的降解以及生长中的透明质酸链从膜上释放。透明质酸酶或源自透明质酸的寡糖不影响合成。链以每分钟60个重复单元的速度生长。在长时间合成过程中持续发生新链起始。用β-N-乙酰氨基葡萄糖苷酶和β-葡萄糖醛酸酶对脉冲追踪标记的透明质酸进行消化表明,链在还原端生长。