Fibbi G, Vannucchi S, Cavallini P, Del Rosso M, Pasquali F, Cappelletti R, Chiarugi V
Biochim Biophys Acta. 1983 Jul 14;762(4):512-8. doi: 10.1016/0167-4889(83)90054-x.
Glycosaminoglycans isolated from native non-adhesive surfaces of both endothelial and mesothelial origin and from endothelial cells cultured in vitro were analyzed by electrophoresis and characterized by chemical and enzymatic breakdown. All the surfaces examined expose in vivo chondroitin 6-sulphate as the main glycosaminoglycan. Under in vitro culture, the exposure of chondroitin sulphate is reduced. Paper chromatography of hydrolysis products upon degradation by chondroitinase AC shows equal amounts of both 6- and 4-sulphated disaccharides. At the same time, the surfaces lose their non-adhesiveness to leukocytes. The addition of fibroblast growth factor to endothelial monolayers restores both non-adhesiveness to leukocytes and exposure of chondroitin sulphate. These results seem to indicate that the exposure of chondroitin sulphate is important in preventing cellular adhesion.
对从内皮和间皮来源的天然非黏附表面以及体外培养的内皮细胞中分离出的糖胺聚糖进行了电泳分析，并通过化学和酶解进行了表征。所有检测的表面在体内均以硫酸软骨素6-硫酸酯作为主要糖胺聚糖。在体外培养条件下，硫酸软骨素的暴露减少。用软骨素酶AC降解后水解产物的纸层析显示，6-硫酸化和4-硫酸化二糖的量相等。同时，这些表面对白细胞失去了非黏附性。向内皮单层细胞中添加成纤维细胞生长因子可恢复对白细胞的非黏附性以及硫酸软骨素的暴露。这些结果似乎表明硫酸软骨素的暴露在防止细胞黏附中很重要。
