人血浆胸腺因子对人外周血单个核细胞亚群的影响。

The effect of a human plasma thymic factor on human peripheral blood mononuclear cell subpopulations.

作者信息

Levai J S, Utermohlen V

出版信息

Clin Immunol Immunopathol. 1983 Jun;27(3):433-43. doi: 10.1016/0090-1229(83)90095-8.

Abstract

Human plasma thymic factor in vitro (human "facteur thymique sérique," or FTS) (at concentrations between 0.25 and 0.25 X 10(5) pg/ml) significantly increased "avid" E-rosette formation by human peripheral blood mononuclear cells (PBMC). It also increased the percentage of OKT8+ cells but did not affect the percentages of OKT3+, OKT4+, OKT11+, OKIa1+, or OKM+ cells. In the low-density immature PBMC, FTS increased both total and avid E-rosette formation, while increasing the percentages of OKT3+ and OKT8+ cells and decreasing the percentage of OKT4+ cells. Theophylline decreased E-rosette formation and the percentages of OKT3+ and OKT4+ among PBMC, and increased the percentages of OKT3+ and OKT4+ among the low-density cells. Human FTS may be capable of inducing maturation of immature PBMC into E-rosette-forming OKT3+8+ cells, while increasing the percentage of mature cells which may be doubly labeled OKT4+8+. The function of such doubly labeled cells remains to be determined. Human FTS may act by increasing intracellular cAMP in immature cells, but probably has a different mode of action in mature cells.

摘要

人血浆胸腺因子体外实验（人“血清胸腺因子”，即FTS）（浓度在0.25至0.25×10⁵皮克/毫升之间）显著增加人外周血单个核细胞（PBMC）的“活性”E花环形成。它还增加了OKT8⁺细胞的百分比，但不影响OKT3⁺、OKT4⁺、OKT11⁺、OKIa1⁺或OKM⁺细胞的百分比。在低密度未成熟PBMC中，FTS增加了总E花环形成和活性E花环形成，同时增加了OKT3⁺和OKT8⁺细胞的百分比，降低了OKT4⁺细胞的百分比。茶碱降低了PBMC中E花环形成以及OKT3⁺和OKT4⁺细胞的百分比，并增加了低密度细胞中OKT3⁺和OKT4⁺细胞的百分比。人FTS可能能够诱导未成熟PBMC成熟为形成E花环的OKT3⁺8⁺细胞，同时增加可能为双重标记OKT4⁺8⁺的成熟细胞的百分比。这种双重标记细胞的功能尚待确定。人FTS可能通过增加未成熟细胞内的环磷酸腺苷（cAMP）起作用，但在成熟细胞中可能具有不同的作用方式。