Webb C F, Gathings W E, Cooper M D
Eur J Immunol. 1983 Jul;13(7):556-9. doi: 10.1002/eji.1830130708.
To test the hypothesis that gamma 3 is the pivotal isotype for sequential heavy chain switching from mu to each of the gamma isotypes, we have compared the effects of anti-gamma 3 and anti-mu antibodies on the expression of immunoglobulin isotypes in lipopolysaccharide (LPS)-stimulated cultures of mouse spleen cells. IgM-, IgG1-, IgG2b- and IgG2a-containing plasma cells were enumerated by immunofluorescence and secreted immunoglobulins were measured by radioimmunoassay. Although anti-gamma 3 and anti-mu were equally effective in inhibiting the LPS-induced differentiation of IgG3 plasma cells, anti-gamma 3 had no effect on the differentiation of IgM, IgG1, IgG2b, or IgG2a plasma cells. These results support a direct mechanism of heavy chain immunoglobulin switching.
为了验证γ3是重链从μ依次转换为各γ同种型的关键同种型这一假说，我们比较了抗γ3抗体和抗μ抗体对脂多糖（LPS）刺激的小鼠脾细胞培养物中免疫球蛋白同种型表达的影响。通过免疫荧光法计数含IgM、IgG1、IgG2b和IgG2a的浆细胞，并通过放射免疫测定法测量分泌的免疫球蛋白。尽管抗γ3和抗μ在抑制LPS诱导的IgG3浆细胞分化方面同样有效，但抗γ3对IgM、IgG1、IgG2b或IgG2a浆细胞的分化没有影响。这些结果支持重链免疫球蛋白转换的直接机制。
