Djovkar A, Hörl W H, Heidland A
Nephron. 1983;34(3):164-7. doi: 10.1159/000183003.
Isolated hindquarters of bilaterally nephrectomized and sham-operated rats were perfused in the presence and absence of 14C-labelled serine, respectively. After a perfusion period of 30 min 14C-serine was 4,074 +/- 270 dpm/ml in the perfusion medium of sham-operated animals and decreased to 2,800 +/- 190 dpm/ml in the medium of acutely uraemic rats. Muscle glycogen concentration in sham-operated animals was 1.10 +/- 0.04 mg/g wet weight in the absence and 1.03 +/- 0.11 mg/g in the presence of serine. In contrast, in acutely uraemic rats there was a glycogen concentration of 0.57 +/- 0.09 mg/g in the absence of serine. Glycogen was increased in the presence of serine in the perfusion medium, the value being 1.50 +/- 0.13 mg glycogen/g wet weight. Incorporation of labelled serine into skeletal muscle glycogen was significantly higher in acutely uraemic animals (15 +/- 0.5 mumol/g glycogen) than in sham-operated animals (10 +/- 0.4 mumol/g). The results are compatible with the hypothesis that serine increases muscle glycogen synthesis in acute uraemia.
分别在有和没有14C标记丝氨酸的情况下，对双侧肾切除和假手术大鼠的离体后肢进行灌注。灌注30分钟后，假手术动物灌注介质中14C-丝氨酸为4,074±270 dpm/ml，而急性尿毒症大鼠介质中则降至2,800±190 dpm/ml。在没有丝氨酸的情况下，假手术动物的肌肉糖原浓度为1.10±0.04 mg/g湿重，有丝氨酸时为1.03±0.11 mg/g。相比之下，急性尿毒症大鼠在没有丝氨酸时糖原浓度为0.57±0.09 mg/g。灌注介质中有丝氨酸时糖原增加，值为1.50±0.13 mg糖原/g湿重。急性尿毒症动物中标记丝氨酸掺入骨骼肌糖原的量（15±0.5 μmol/g糖原）显著高于假手术动物（10±0.4 μmol/g）。这些结果与丝氨酸增加急性尿毒症时肌肉糖原合成的假说相符。
