Gundersen S, Funderud S, Bloom B R, Godal T
Acta Pathol Microbiol Immunol Scand C. 1983 Apr;91(2):137-45.
A correlation between natural killer (NK) cell activity and growth inhibition (GI), as measured in an in vitro assay with B16 melanoma cells, mediated by a serum growth-inhibitory factor (GIF), among various strains of mice has been demonstrated. Beige mice (bg/bg), known to express low NK-activity, were also low in GIF activity and showed increased susceptibility to both B16 melanoma cells and another solid tumor (Lewis lung carcinoma) in vivo. In vivo treatment with various protease inhibitors that reduced NK-activity, also reduced growth inhibition mediated by GIF. Protease inhibitors that did not affect NK-activity did not affect GIF either. All of 4 B16 melanoma clones selected against GIF in vitro and resistant to GIF showed both resistance to NK-cells in vitro and increased growth potential in vivo. However, P-815, an NK-resistant cell line, was sensitive to GIF.
在一项体外实验中，利用血清生长抑制因子（GIF）对不同品系小鼠进行检测，结果表明自然杀伤（NK）细胞活性与生长抑制（GI）之间存在相关性，该实验采用B16黑色素瘤细胞进行。已知米色小鼠（bg/bg）表达的NK活性较低，其GIF活性也较低，并且在体内对B16黑色素瘤细胞和另一种实体瘤（Lewis肺癌）的易感性增加。用各种降低NK活性的蛋白酶抑制剂进行体内治疗，也会降低GIF介导的生长抑制。不影响NK活性的蛋白酶抑制剂也不会影响GIF。在体外针对GIF筛选出的4个B16黑色素瘤克隆，且对GIF具有抗性，它们在体外对NK细胞具有抗性，并且在体内具有更高的生长潜力。然而，P - 815是一种对NK具有抗性的细胞系，对GIF敏感。
