Gómez Torres R, Rodríquez Vázquez M L, Mooser Barandum O, Ramírez Isunza J M
Arch Inst Cardiol Mex. 1983 Mar-Apr;53(2):139-42.
In 1974, two adult specimens of triatoma were obtained in the municipality of Calvillo, Aguascalientes. Tripomastigotes were found by studying their excreta in the microscope. Since no reports of naturally infected triatoma were reported from Aguascalientes, we decided to further study this finding. Additional triatoma specimens were collected from this municipality and their excreta was infected to albino mice. Twenty one days later the animals had parasitemia and with tripomastigotes, some of them died. The survivors were sacrificed and their heart was obtained for histological sections all of them had nests of amastigotes. Cultures were performed from the blood of infected animals employing as a nest NNN (Novy, Mc Neal, Nicolle), and epimastigotes and tripomastigotes grew. The lesions found in the histological study, the evolutive forms of the parasite in the transmitting insect and the host, strongly suggest the presence of trypanosoma cruzi.
1974年,在阿瓜斯卡连特斯州卡尔维洛市获得了两只成年锥蝽标本。通过在显微镜下研究它们的排泄物发现了锥鞭毛体。由于阿瓜斯卡连特斯州此前没有自然感染锥蝽的报告,我们决定进一步研究这一发现。从该市收集了更多的锥蝽标本,并将它们的排泄物接种给白化小鼠。二十一天后,这些动物出现了寄生虫血症并带有锥鞭毛体,其中一些死亡。对存活的动物实施安乐死并获取其心脏进行组织切片检查,发现所有动物的心脏中都有无鞭毛体巢。用NNN(诺维、麦克尼尔、尼科尔)培养基对感染动物的血液进行培养,长出了上鞭毛体和锥鞭毛体。组织学研究中发现的病变、传播昆虫和宿主体内寄生虫的发育形态,强烈提示存在克氏锥虫。