乳酸脱氢酶和麦胚凝集素在带有羧基功能的新型凝胶上的亲和层析。
Affinity chromatography of lactate dehydrogenase and wheat germ lectin on new gels bearing carboxylic functions.
作者信息
Brown E, Joyeau R, Boschetti E, Moroux Y
出版信息
J Chromatogr. 1978 Mar 11;150(1):110-7.
PMID:641113
Abstract
The suitability of two new functionalized copolymer gels for use in affinity chromatography has been examined. Both gels were substituted by two ligands, one being specific for lactate dehydrogenase and the other for wheat germ lectin. The derivatives thus obtained were used successfully for the purification of two proteins with different biological activities.
摘要
已对两种用于亲和色谱的新型功能化共聚物凝胶的适用性进行了研究。两种凝胶都被两种配体取代，一种对乳酸脱氢酶具有特异性，另一种对麦胚凝集素具有特异性。由此获得的衍生物已成功用于纯化两种具有不同生物活性的蛋白质。
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