Levine D Z, McLeod R A, Raman S
Can J Physiol Pharmacol. 1983 Jun;61(6):641-6. doi: 10.1139/y83-098.
Studies have been carried out in awake chronically cannulated adrenalectomized rats subjected to steroid replacement protocols. Our objective was to determine the effects of mineralocorticoid or glucocorticoid or combined replacement on acid-base composition of the extracellular fluid compartment in response to an acute ammonium chloride acid load. We obtained six blood samples from each animal over a 26-h period and, with the help of a specially derived mathematical model, we were able to obtain a continuous dynamic assessment of changes of plasma bicarbonate concentration during induction and recovery of the metabolic acidosis. Assessment of minimum values for plasma bicarbonate concentration, time to reach the minima, and time for 90% recovery showed that dexamethasone did not exert as much protection as aldosterone. The approach we have used, in a sense being a continuous display of plasma bicarbonate concentration, should be useful in further exploring the nature of the interrelations between acid production, net acid excretion, and the resultant plasma acid-base composition.
研究在接受类固醇替代方案的清醒慢性插管肾上腺切除大鼠中进行。我们的目的是确定盐皮质激素或糖皮质激素或联合替代对细胞外液区酸碱组成的影响，以应对急性氯化铵酸负荷。在26小时内从每只动物获取六份血样，并借助一个专门推导的数学模型，我们能够对代谢性酸中毒诱导和恢复期间血浆碳酸氢盐浓度的变化进行连续动态评估。对血浆碳酸氢盐浓度的最小值、达到最小值的时间以及90%恢复时间的评估表明，地塞米松的保护作用不如醛固酮。我们所采用的方法，从某种意义上来说是血浆碳酸氢盐浓度的连续展示，应该有助于进一步探索产酸、净酸排泄与由此产生的血浆酸碱组成之间相互关系的本质。
